Descripción
The frozen collection consists of over 3,000 transgenic and mutant strains of non-parasitic nematodes, Caenorhabditis elegans species, accumulated over forty years. The collection, established in the early ’80s by Dr. Paolo Bazzicalupo, is kept in triplicate, is unique in Italy, and supports numerous Italian groups. C. elegans is the only multicellular animal model that can be frozen and stabs are stable for decades.
Preservation techniques: Cryopreservation of L1 larval stage worms in 1 mL freezing solution containing NaCl 0.1M, phosphate buffer pH 6.2, Glycerol 30%. Services: Distribution of strains to the Italian C. elegans community. Local conservation of strains from CAENORHABDITIS GENETICS CENTER (CGC). Genotyping and phenotyping.
Competence / Expertise: Maintenance of C. elegans strains, Genetic crosses, Molecular biology, Anatomical methods, Neurobiology, Toxicology, Implementation of nutraceuticals, Genetic screens, Drug screens, Transgenesis.
Quality: Genotyping and phenotyping of strains before and after freezing, including viability test. The collection is maintained as triplicate copies in 2 different -80° Ultrafreezers and 1 in L.N.
Registros
Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 417 registros.
también existen 2 tablas de datos de extensiones. Un registro en una extensión provee información adicional sobre un registro en el core. El número de registros en cada tabla de datos de la extensión se ilustra a continuación.
Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Di Schiavi E, Zampi G, Bazzicalupo P (2023). C. elegans Italian collection (CelITAbase) (CNR-IBBR-CELITABASE). Version 1.0. GBIF Secretariat. Occurrence dataset. https://www.gbif.org/dataset/
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es Consiglio Nazionale delle Ricerche, Istituto di Bioscienze e BioRisorse (CNR-IBBR). Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento-NoComercial (CC-BY-NC 4.0).
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: 0e6c4d5b-5156-4792-ba5a-9adb04c5edbe. Consiglio Nazionale delle Ricerche, Istituto di Bioscienze e BioRisorse (CNR-IBBR) publica este recurso y está registrado en GBIF como un publicador de datos avalado por DiSSCo.
Palabras clave
Occurrence; Genetics; animal model; invertebrate; non parasitic nematode; Specimen
Datos externos
Los datos del recurso también están disponibles en otros formatos
| API-JSON | https://biomemory.cnr.it/api/occurrences/json/?dataset=141 UTF-8 DwC |
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Contactos
- Punto De Contacto
- Senior Researcher
- v. Pietro Castellino 111
https://orcid.org/0000-0002-8179-6666
- Originador
- Collection manager
- v. Pietro Castellino 111
- Originador
- Retired research Director
- v. Pietro Castellino 111
- Proveedor De Los Metadatos
- Senior Researcher
- v. Madonna del Piano 10
- Punto De Contacto
- Reference Person
- v. Pietro Castellino 111
- Proveedor De Contenido
- Research Department
- p.le Aldo Moro 7
Cobertura geográfica
Worldwide
| Coordenadas límite | Latitud Mínima Longitud Mínima [-90, -180], Latitud Máxima Longitud Máxima [90, 180] |
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Cobertura taxonómica
Caenorhabditis elegans
| Especie | Caenorhabditis elegans |
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Cobertura temporal
| Periodo de formación | 2002-2024 |
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Datos del proyecto
ITINERIS (PNRR Project IR0000032) Activity 6.5 "Mining and mapping the functional biodiversity in vivo and ex-situ research collections" - This activity is aimed to provide biodiversity (meta)data linked to Natural Science Collections (NSC) in terms of extended digital specimens fully linked to related information (e.g.. images and videos, codification of functional traits, DNA and protein sequences, environmental parameters, etc.). The expected outcome of the activity is the implementation of a national network of biological research collections, including all the existing in vivo and ex-situ collections of biological organisms (animals, plants, fungi, algae, archaea, bacteria, viruses, etc.) which have been created and maintained through past and current research projects. The full digitization of the research collections currently offline will be achieved along with the reorganization, standardization and harmonization of the data and metadata currently available in the main online collections. All the taxonomic, genetic, geographical, ecological, physiological information for each specimen will be organized in an easily searchable platform supported by web-GIS functionalities. Reference to genetic/genomic data for each specimen will allow for the identification of the most interesting material for further genetic/genomic analysis. The availability of such huge amount of information on plant, animal, and microbial genetic resources will pave the way for the dissection of the morpho-physiological and molecular mechanisms of adaptation to climate change. || BIOMEMORY (CNR-DiSBA Project No. SAC.AD002.173) is the network of biological collections of the Department of Biology, Agriculture and Food Sciences (DiSBA) of the National Research Council of Italy (CNR) for bio-monitoring, biodiversity conservation, agri-food and environmental sustainability, and human well-being. The project is aimed to create a network of biobanks (i.e., scientific research collections) where data and metadata associated to biological samples of different nature are collected and stored in a systematic and well-organized way. Maintaining the existing collections will allow their future use for a number of purposes, from the genetic improvement of organisms to face environmental changes (climate-ready organisms) to the fight against epidemics and pandemics affecting humans, animals and plants.
| Título | ITINERIS - Italian Integrated Environmental Research Infrastructures System / BIOMEMORY |
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| Identificador | PNRR Project IR0000032 || CNR-DiSBA no. SAC.AD002.173 |
| Fuentes de Financiación | ITINERIS project no. IR0000032 — ITINERIS, Italian Integrated Environmental Research Infrastructures System—CUP B53C22002150006 (D.D. n. 130/2022) Funded by the EU—Next Generation EU—Mission 4 “Education and Research”—Component 2: “From research to business”—Investment 3.1: “Fund for the realisation of an integrated system of research and innovation infrastructures” || BIOMEMORY (CNR-DiSBA Project No. SAC.AD002.173 |
| Project Award |
BioMemory Project (CNR-DiSBA) https://doi.org/10.13039/501100004462 Consiglio Nazionale delle Ricerche CNR-DiSBA no. SAC.AD002.173 https://www.disba.cnr.it/en/ ITINERIS - Italian Integrated Environmental Research Infrastructures System https://doi.org/10.13039/501100004462 Ministero dell'Università e della Ricerca PNRR Project IR0000032 https://itineris.cnr.it/ |
| Related Projects |
Multicentre Research Grant 656IT/cv2008.1224 from Compagnia di Sanpaolo, “Bando 2008 del Programma di Neuroscienze” MERIT Grant RBNE08LN4P_002 from CNR/MIUR Grant "FaReBio di Qualità - Farmaci e reti Biotecnologiche di Qualità" from the Italian Ministry of Economy Grant "BANDO RICERCA FINALIZZATA 2009" RF-2009-1475235 from the Italian Ministry of Health Grant Misura 3.17-2008 from Regione Campania, Italy Grant Exploratory Project GEP14141 from Italian Telethon Foundation Grant "BANDO RICERCA SCIENTIFICA E TECNOLOGICA 2015" 2015.03003.021 from Fondazione Cassa di Risparmio di Perugia, Italy Grant "BeHEARD Technology Prizes Rare Disease Challenge" Grant Full Project GGP16203 from Italian Telethon Foundation Grant H2020-FETOPEN-1-2016-2017 801338 from European Community Grant Joint Bilateral Agreement CNR/RFBR from National Research Council of Italy (CNR) Grant SMA-Europe Operating Grant 2019 22757 Grant H2020-FETOPEN-PROACTIVE-EIC-05 NUMBER 952183 from European Community Grant CHARGE Syndrome Foundation Research Grant Program 2021 from Charge Foundation Research Grant AFM-TELETHON ANNUAL CALL FOR PROPOSALS 2022 from AFM-Telethon France |
Personas asociadas al proyecto:
Datos de la colección
| Nombre de la Colección | Caenorhabditis elegans Italian collection (CelITAbase) |
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| Identificador de la Colección | 1d38cf8c-78a6-411e-b574-533dc840b43c |
| Métodos de preservación de los ejemplares | Congelado |
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Metadatos adicionales
| Agradecimientos | PNRR Project IR0000032—ITINERIS, Italian Integrated Environmental Research Infrastructures System—CUP B53C22002150006 (D.D. n. 130/2022) Funded by the EU—Next Generation EU—Mission 4 “Education and Research”—Component 2: “From research to business”—Investment 3.1: “Fund for the realization of an integrated system of research and innovation infrastructures” CNR-DiSBA Project No. SAC.AD002.173 BioMemory - Network of scientific collections for bio-monitoring, biodiversity conservation, agri-food and environmental sustainability, and well-being. |
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| Propósito | Not-for-profit use only. Without our written consent, all strains may not be redistributed outside the recipient’s organization. Some strains can be distributed by accepting to cite the paper in which it has been described. Some strains contain patented and/or exclusively licensed components and can be distributed only under a collaborative agreement. |
| Descripción de mantenimiento | Cryopreservation of L1 larval stage worms in 1 mL freezing solution containing NaCl 0.1M, phosphate buffer pH6.2, Glycerol 30%. |
| Identificadores alternativos | https://ipt.ibbr.cnr.it/ipt/resource?r=celitabase-01 |